Figure 2: Genomic and complementary DNA, and protein structures of the human (h) GR, functional distribution of the GR, and the isoforms produced through alternative splicing.
The hGR gene consists of 10 exons. Exon 1 is untranslated region, exon 2 codes for the immunogenic domain (A/B), exon 3 and 4 for the DNA-binding domain (C), and exons 5-9 for the hinge region (D) and the ligand-binding domain (E). GR does not contain an F region in contrast to the other steroid hormone receptors. The GR gene contains two terminal exons 9 (exon 9 and 9) alternatively spliced to produce the classic GR and the nonligand-binding GR. C-terminal gray colored domains in GR and GR show their specific portions. Locations of several functional domains are also indicated.
AF-1 and -2: activation function 1 and 2; DBD; DNA-binding domain; HD: hinge region; LBD: Ligand-binding domain; NTD: N-terminal region, NL1 and 2: Nuclear translocation signal 1 and 2.