Figure 15: Clock/Bmal1 suppresses GR-induced transcriptional activity through acetylation.
Clock physically interacts with GR LBD through its nuclear receptor-interacting domain and suppresses GR-induced transcriptional activity by acetylating with its intrinsic HAT activity a lysine cluster located in the hinge region of the GR (A) through which Clock reduces affinity of GR to its cognate DNA GREs (B).
A: acetylation; Bmal1: brain-muscle-arnt-like protein 1; DBD: DNA-binding domain; GREs: glucocorticoid response elements; HR: hinge region; K: lysine residue; LBD: ligand-binding domain; NTD: N-terminal domain
From (238).