etx-dm-ch3-fig4

Figure 4. Architectural assembly of the unbound insulin receptor ectodomain. This figure is based on the latest higher resolution structure of the apo-receptor at 3.3 Å resolution (38). A. The α subunit. Domains are labeled as in Figure 3. FnIII-2α is the α subunit component of the FnIII-2 domain. B. The β subunit. Domains are labeled as in Figure 3. FnIII-2β is the β subunit component of the FnIII-2 domain. C. The αβ monomer. The monomer shows an inverted V-shaped structure. The arrow denotes the site of proteolytic cleavage of the proreceptor. This structure is the A-isoform of the insulin receptor. Twelve amino acids at the end of the insert domain of the β subunit are missing from the structure. The B-isoform would have 12 more amino acids encoded by exon 11 at the end of the αCT domain of the α subunit..D. The α2β2 dimer. The arrow indicates the location of the triplet of disulfide bonds between Cys 682, 683 and 685 of the two α subunits. E. The tandem insulin binding site 1 made of the αCT helical segment of one α subunit binding in trans to the beta sheet surface of the L1 domain of the second α subunit. The rest of the α subunit insert (ID), which is not part of the binding site, is also shown for orientation. F. Idem, rotated 90 degrees. Drawn using DSViewerPro from PDB file Model-S1 2, kindly provided by Mike Lawrence, based on PDB file 4ZXB complemented using IMDFF (38).

Figure 4. Architectural assembly of the unbound insulin receptor ectodomain. This figure is based on the latest higher resolution structure of the apo-receptor at 3.3 Å resolution (38). A. The α subunit. Domains are labeled as in Figure 3. FnIII-2α is the α subunit component of the FnIII-2 domain. B. The β subunit. Domains are labeled as in Figure 3. FnIII-2β is the β subunit component of the FnIII-2 domain. C. The αβ monomer. The monomer shows an inverted V-shaped structure. The arrow denotes the site of proteolytic cleavage of the proreceptor. This structure is the A-isoform of the insulin receptor. Twelve amino acids at the end of the insert domain of the β subunit are missing from the structure. The B-isoform would have 12 more amino acids encoded by exon 11 at the end of the αCT domain of the α subunit..D. The α2β2 dimer. The arrow indicates the location of the triplet of disulfide bonds between Cys 682, 683 and 685 of the two α subunits. E. The tandem insulin binding site 1 made of the αCT helical segment of one α subunit binding in trans to the beta sheet surface of the L1 domain of the second α subunit. The rest of the α subunit insert (ID), which is not part of the binding site, is also shown for orientation. F. Idem, rotated 90 degrees. Drawn using DSViewerPro from PDB file Model-S1 2, kindly provided by Mike Lawrence, based on PDB file 4ZXB complemented using IMDFF (38).

Figure 4. Architectural assembly of the unbound insulin receptor ectodomain. This figure is based on the latest higher resolution structure of the apo-receptor at 3.3 Å resolution (38). A. The α subunit. Domains are labeled as in Figure 3. FnIII-2α is the α subunit component of the FnIII-2 domain. B. The β subunit. Domains are labeled as in Figure 3. FnIII-2β is the β subunit component of the FnIII-2 domain. C. The αβ monomer. The monomer shows an inverted V-shaped structure. The arrow denotes the site of proteolytic cleavage of the proreceptor. This structure is the A-isoform of the insulin receptor. Twelve amino acids at the end of the insert domain of the β subunit are missing from the structure. The B-isoform would have 12 more amino acids encoded by exon 11 at the end of the αCT domain of the α subunit..D. The α2β2 dimer. The arrow indicates the location of the triplet of disulfide bonds between Cys 682, 683 and 685 of the two α subunits. E. The tandem insulin binding site 1 made of the αCT helical segment of one α subunit binding in trans to the beta sheet surface of the L1 domain of the second α subunit. The rest of the α subunit insert (ID), which is not part of the binding site, is also shown for orientation. F. Idem, rotated 90 degrees. Drawn using DSViewerPro from PDB file Model-S1 2, kindly provided by Mike Lawrence, based on PDB file 4ZXB complemented using IMDFF (38).